HPLC vs. LC-MS in Peptide Testing
This resource is educational documentation for laboratory procurement and record review. It does not provide medical advice, treatment guidance, dosage, administration, or reconstitution instructions.
HPLC is commonly used to separate and monitor compounds by a detector such as UV. LC-MS couples liquid chromatography with mass spectrometry, adding mass-to-charge information to the chromatographic separation. The NCBI chromatography reference and NCBI LC-MS reference provide technical background.
Neither method alone proves sterility, endotoxin control, manufacturing grade or suitability outside the documented laboratory-research context. A documentation matrix should show the exact method performed and should not substitute one method for another.
Research Use Only. Not for human or animal consumption, diagnostic use, therapeutic use, veterinary use, or clinical application.